Eugon LT 100 Broth

Eugon LT 100 Broth E
Art. No. 02-654

Specification Diluent). Immediately transfer the membrane to a suitable volume of the
Eugon broth. The inoculated broth is incubated at 32,5 ± 2,5ºC for 20-72
Liquid medium used for the enrichment of aerobic bacteria including
hours.
E. coli, in cosmetic products with and without preservatives according
If enumeration by the MPN method is being carried out proceed as
to ISO standards.
follows:
Prepare serial tenfold dilutions bank of the sample. Inoculate, incubate
Formula* in g/L and enumerate as per the Most Probable Number Protocol. Carry out
enumeration according to the appropriate tables in each case.
Tryptone 15,00
References
Soy peptone 5,00
Polysorbate 80 5,00
Dextrose 5,50 · GUISNO, R., I.W. GIBBY & M.J. FOTER (1946) A neutralizing medium for
Sodium chloride 4,00 evaluation of the germicidal potency of the quaternary ammonium salts.
Lecithin 1,00 Amer. J. Pharm. 118:320-323.
Triton® X-100 1,00 · ISO 21149 Standard (2006) Cosmetics - Microbiology - Enumeration
L-Cysteine HCl 0,70 and detection of aerobic mesophilic bacteria.
Sodium sulfite 0,20 · ISO 21150 Standard (2006) Cosmetics - Microbiology - Detection of
Final pH 7,0 ± 0,2 at 25ºC Escherichia coli.
* Adjusted and /or supplemented as required to meet performance criteria · ISO/TS 11133-1: 2009 Microbiology of food and animal feeding stuffs.-
Guidelines on preparation and production of culture media. Part 1:
Directions General guidelines on quality assurance for the preparation of culture

media in the laboratory.
Dissolve 37,4 g of powder in 1 L of distilled water. Bring to the boil gently · ISO/TS 11133-2: 2003 Corr. 2004 Microbiology of food and animal
and distribute into suitable containers. Sterilize in the autoclave at 121ºC feeding stuffs.- Guidelines on preparation and production of culture
for 15 minutes. Cool rapidly. The medium will become clear on cooling. media. Part 2: Practical guidelines on performance testing of culture
Note: The lumpy and greasy aspect of the dehydrated powder is normal media.
due to the polysorbate and Triton® components and does not affect its · WILLIAMSON, P. & A.M. KLIGMAN (1965) A new method for the
performance. quantitative investigation of cutaneous bacteria. J. Inv. Dermatol.
45:498-503
Description
Storage
Eugon LT 100 Broth is a general culture medium that allows the growth of
aerobic, and microaerophilic bacteria. Some anaerobic microorganisms For laboratory use only. Keep tightly closed, away from bright light, in a
will grow, due to the low Eh potential generated by the cysteine and cool dry place (+4ºC to 30ºC and <60% RH).
sodium sulfite components.
The main use of this medium is for the total enumeration of microorganisms (continues on the next page)
in cosmetic products by the Most Probable Number (MPN) method.
The Triton® X-100 included in the formula enhances the release of
microorganisms from the slack matrix of the cosmetic emulsion.
Lecithin and polysorbate act as neutralizers of preservatives like
quaternary ammonium compounds, phenol and aldehydes derivatives.
Technique
A 1:10 dilution of the sample is prepared using directly Eugon broth if
the sample is soluble in water. If the sample is non-water soluble, it must
emulsified with a suitable agent (e.g. Polysorbate 80). Once emulsified
the sample is added to a suitable volume of Eugon broth (e.g. 1:10). If the
sample is filterable it is recommended filtering it through a membrane
filter with a nominal pore no greater than to 0.45 μm and washing it with
defined volumes of water or diluent (Art. No. 02-510 Maximum Recovery
www.scharlab.com
scharlab@scharlab.com Scharlau, The wise choice 161
E Eugon LT 100 Broth
Art. No. 02-654
Quality control
Incubation temperature: 32,5 ± 2,5ºC
Incubation time: 24 h
Inoculum: 10-100 CFU (Productivity) according to ISO 11133-1/2
Microorganism Growth Remarks
Staphylococcus aureus ATCC 25923 Good -
Staphylococcus epidermidis ATCC 12228 Good -
Escherichia coli ATCC 25922 Good -
Pseudomonas aeruginosa ATCC 27853 Good -
Salmonella typhimurium ATCC 14028 Good -
www.scharlab.com
162 Scharlau, The wise choice scharlab@scharlab.com

Eugon LT 100 Broth

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Eugon LT 100 Broth E

Art. No. 02-654

Directions General guidelines on quality assurance for the preparation of culture

Microorganism Growth Remarks

Staphylococcus aureus ATCC 25923 Good -

Staphylococcus epidermidis ATCC 12228 Good -

Escherichia coli ATCC 25922 Good -

Pseudomonas aeruginosa ATCC 27853 Good -

Salmonella typhimurium ATCC 14028 Good -

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